Coupled transcription and translation within nuclei of mammalian cells.

Journal article

It is widely assumed that the vital processes of transcription and translation are spatially separated in eukaryotes and that no translation occurs in nuclei. We localized translation sites by incubating permeabilized mammalian cells with [3H]lysine or lysyl-transfer RNA tagged with biotin or BODIPY; although most nascent polypeptides were cytoplasmic, some were found in discrete nuclear sites known as transcription "factories." Some of this nuclear translation also depends on concurrent tran...

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Authors: Iborra, F; Jackson, D; Cook, P

• Publication status: Published
• Journal: Science (New York, N.Y.)
• Volume: 293
• Issue: 5532
• Pages: 1139-1142
• Publication date: 2001-08-01
• DOI: 10.1126/science.1061216
• EISSN: 1095-9203
• ISSN: 0036-8075
• Language: English
• Keywords: COS Cells; Immunohistochemistry; Animals; Transcription, Genetic; Ribosomes; Tumor Cells, Cultured; Protein Biosynthesis; Cell Fractionation; Biotin; Hela Cells; Proteins; RNA Polymerase II; Protein Transport; Fluorescence; Cytoplasm; Humans; Mitochondria; Autoradiography; Cell Membrane Permeability; Cycloheximide; Cell Nucleus; Protein Synthesis Inhibitors; Boron Compounds; RNA, Transfer, Amino Acyl