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Purification to homogeneity of Charonia lampas alpha-fucosidase by using sequential ligand-affinity chromatography.

Abstract:

An alpha-fucosidase from the liver of the marine gastropod Charonia lampas was purified to homogeneity using a procedure that included cation-exchange and gel-filtration chromatography, chromatofocusing and a final series of affinity-chromatography steps which involved the following gel-immobilized ligands: N-(5-carboxy-1-pentyl)-1,5-dideoxy-1,5-imino-L-fucitol, N-(5-carboxy-1-pentyl)-2-acetamido-1,5-imino-1,2,5-trideoxy-D-glucitol and thio-beta-D-galactoside. The enzyme was found to be a tet...

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Publication status:
Published

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Authors


Butters, TD More by this author
Scudder, P More by this author
Rotsaert, J More by this author
Petursson, S More by this author
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Journal:
The Biochemical journal
Volume:
279 ( Pt 1)
Issue:
1
Pages:
189-195
Publication date:
1991-10-05
EISSN:
1470-8728
ISSN:
0264-6021
URN:
uuid:b485941e-0aab-4e75-95ef-634e10c704ba
Source identifiers:
43652
Local pid:
pubs:43652

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