Purification to homogeneity of Charonia lampas alpha-fucosidase by using sequential ligand-affinity chromatography.

Journal article

An alpha-fucosidase from the liver of the marine gastropod Charonia lampas was purified to homogeneity using a procedure that included cation-exchange and gel-filtration chromatography, chromatofocusing and a final series of affinity-chromatography steps which involved the following gel-immobilized ligands: N-(5-carboxy-1-pentyl)-1,5-dideoxy-1,5-imino-L-fucitol, N-(5-carboxy-1-pentyl)-2-acetamido-1,5-imino-1,2,5-trideoxy-D-glucitol and thio-beta-D-galactoside. The enzyme was found to be a tet...

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Authors: Butters, T; Scudder, P; Rotsaert, J; Petursson, S; Fleet, G

• Publication status: Published
• Journal: Biochemical journal
• Volume: 279 ( Pt 1)
• Issue: 1
• Pages: 189-195
• Publication date: 1991-10-01
• EISSN: 1470-8728
• ISSN: 0264-6021
• Language: English
• Keywords: Isoelectric Focusing; Parotid Gland; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Polysaccharides; Mollusca; Hydrolysis; alpha-L-Fucosidase; Kinetics; Liver; Oligosaccharides; Animals