- Abstract:
-
The enzymatic dissociation of acinar tissue by collagenase is a substantial step in the isolation of pancreatic islets. Although essential collagenase components have been purified, the variability in the activity of different batches limits long-term reproducibility of isolation success. The utilization of purified recombinant proteases would solve this problem. In the present study, pancreases from multiorgan donors were dissociated by means of digestion-filtration using either Liberase HI ...
Expand abstract - Publisher copy:
- 10.2337/diabetes.52.5.1143
-
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- Journal:
- Diabetes
- Volume:
- 52
- Issue:
- 5
- Pages:
- 1143-1146
- Publication date:
- 2003-05-05
- DOI:
- EISSN:
-
1939-327X
- ISSN:
-
0012-1797
- URN:
-
uuid:b404628f-e1cd-48b5-9144-21111205983c
- Source identifiers:
-
262704
- Local pid:
- pubs:262704
- Language:
- English
- Keywords:
- Copyright date:
- 2003
Journal article
Successful human islet isolation utilizing recombinant collagenase.
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