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Highly multiplexed single-cell analysis of formalin-fixed, paraffin-embedded cancer tissue.

Abstract:

Limitations on the number of unique protein and DNA molecules that can be characterized microscopically in a single tissue specimen impede advances in understanding the biological basis of health and disease. Here we present a multiplexed fluorescence microscopy method (MxIF) for quantitative, single-cell, and subcellular characterization of multiple analytes in formalin-fixed paraffin-embedded tissue. Chemical inactivation of fluorescent dyes after each image acquisition round allows reuse o...

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Publisher copy:
10.1073/pnas.1300136110

Authors


Gerdes, MJ More by this author
Sevinsky, CJ More by this author
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Journal:
Proceedings of the National Academy of Sciences of the United States of America
Volume:
110
Issue:
29
Pages:
11982-11987
Publication date:
2013-07-05
DOI:
EISSN:
1091-6490
ISSN:
0027-8424
URN:
uuid:b3f560ca-8bbf-4e01-95d1-77e75f4c9e7f
Source identifiers:
426712
Local pid:
pubs:426712

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