Studies on bone matrix glycoproteins. Incorporation of (1-14C)glucosamine and plasma (14C)glycoprotein into rabbit cortical bone.

Journal article

The radioactively labelled constituents present in bone matrix were compared 12 days after injection of either [(14)C]glucosamine or plasma [(14)C]glycoprotein. Both precursors are utilized in the synthesis of organic matrix by bone tissue. Cortical bone from animals injected with [(14)C]glucosamine contains radioactivity derived from glucosamine and plasma glycoproteins and all glycoprotein fractions are labelled. Plasma [(14)C]glycoprotein labels the less acidic glycoproteins to a greater extent than the more acidic components. An antibody has been raised against the less-acidic-glycoprotein fraction of bone. The latter contains a glycoprotein of alpha-mobility that appears to be concentrated specifically in bone tissue and which is present also in plasma. This alpha-glycoprotein accounts for a large proportion of the components labelled and retained in bone matrix after [(14)C]glucosamine injection.

Authors: Triffitt, J; Owen, M

• Publication status: Published
• Journal: Biochemical journal
• Volume: 136
• Issue: 1
• Pages: 125-134
• Publication date: 1973-09-01
• EISSN: 1470-8728
• ISSN: 0264-6021
• Language: English
• Keywords: Goats; Antibodies; Glycoproteins; Blood Proteins; Serum Albumin; Glucosamine; Blood Volume; Immunoelectrophoresis; Blood Protein Electrophoresis; Microbial Collagenase; Albumins; Carbon Radioisotopes; Sheep; Animals; Immune Sera; Chromatography, DEAE-Cellulose; Rabbits; Electrophoresis, Polyacrylamide Gel; Guinea Pigs; Bone Matrix