Adaptive optics in super-resolution microscopy

Journal article

Super-resolution fluorescence microscopy has entered most biological laboratories worldwide and its benefit is undisputable. Its application to brain imaging, for example in living mice, enables the study of sub-cellular structural plasticity and brain function directly in a living mammal. The demands of brain imaging on the different super-resolution microscopy techniques (STED, RESOLFT, SIM, ISM) and labeling strategies are discussed here as well as the challenges of the required cranial window preparation. Applications of super-resolution in the anesthetized mouse brain enlighten the stability and plasticity of synaptic nanostructures. These studies show the potential of in vivo super-resolution imaging and justify its application more widely in vivo to investigate the role of nanostructures in memory and learning

Authors: Wang, J; Zhang, Y

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Biophysical Society of China
• Journal: Biophysics Reports
• Volume: 7
• Issue: 4
• Pages: 267-279
• Publication date: 2021-01-01
• DOI: 10.52601/bpr.2021.210015
• ISSN: 2364-3420
• Language: English
• Keywords: Fluorescence; Artificial intelligence; Scanning confocal electron microscopy; Super-resolution microscopy; Fluorescence microscope; Diffraction; Computer science; Resolution (logic); Adaptive optics; Light sheet fluorescence microscopy; Physics; Materials science; Nanotechnology; Microscopy; Optics