Critical roles of sepsis-reshaped fecal virota in attenuating sepsis severity

Chancharoenthana, W; Sutnu, N; Visitchanakun, P; Sawaswong, V; Chitcharoen, S; Payungporn, S; Schuetz, A; Schultz, MJ; Leelahavanichkul, A

Journal article

Critical roles of sepsis-reshaped fecal virota in attenuating sepsis severity

Abstract:: Background: Most of the current bacteriophages (phages) are mostly isolated from environments. However, phages isolated from feces might be more specific to the bacteria that are harmful to the host. Meanwhile, some phages from the environment might affect non-pathogenic bacteria for the host. Methods: Here, bacteriophages isolated from mouse feces were intratracheally (IT) or intravenously (IV) administered in pneumonia mice caused by Pseudomonas aeruginosa at 2 hours post-intratracheal bacterial administration. As such, the mice with phage treatment, using either IT or IV administration, demonstrated less severe pneumonia as indicated by mortality, serum cytokines, bacteremia, bacterial abundance in bronchoalveolar lavage fluid (BALF), and neutrophil extracellular traps (NETs) in lung tissue (immunofluorescence of neutrophil elastase and myeloperoxidase). Results: Interestingly, the abundance of phages in BALF from the IT and IV injections was similar, supporting a flexible route of phage administration. With the incubation of bacteria with neutrophils, the presence of bacteriophages significantly improved bactericidal activity, but not NETs formation, with the elevated supernatant IL-6 and TNF-α, but not IL-1β. In conclusion, our findings suggest that bacteriophages against Pseudomonas aeruginosa can be discovered from feces of the host. Conclusions: The phages attenuate pneumonia partly through an enhanced neutrophil bactericidal activity, but not via inducing NETs formation. The isolation of phages from the infected hosts themselves might be practically useful for future treatment. More studies are warranted

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Chancharoenthana, W., Sutnu, N., Visitchanakun, P., Sawaswong, V., Chitcharoen, S., Payungporn, S., Schuetz, A., Schultz, M. J., & Leelahavanichkul, A. (2022). Critical roles of sepsis-reshaped fecal virota in attenuating sepsis severity. Frontiers in Immunology, 13, 940935.

MLA Style

Chancharoenthana, W, et al. “Critical Roles of Sepsis-Reshaped Fecal Virota in Attenuating Sepsis Severity.” Frontiers in Immunology, vol. 13, 2022, p. 940935.

Chicago Style

Chancharoenthana, W, N Sutnu, P Visitchanakun, et al. 2022. “Critical Roles of Sepsis-Reshaped Fecal Virota in Attenuating Sepsis Severity.” Frontiers in Immunology 13: 940935.
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