Separation and characterization of lipopolysaccharide isomers using capillary electrophoresis-electrospray tandem mass spectrometry

Journal article

The electrophoretic separation of trace level lipopolysaccharide (LPS) isoforms and their molecular structure were analyzed using tandem mass spectrometry. A Crystal Model 310 CE instrument was coupled to an API 3000 mass spectrometer via a microlonspray interface. LPS from monoclonal antibody B 5 positive phase variant of N.meningitidis clinical isolates, BZ157 was obtained. Structural informations were obtained using second generation product ions of fragments formed by in-source CID under high orifice voltage conditions. The product ion spectrum corresponding to the isomer of higher electrophoretic mobility showed a neutral loss of 952 Da (m/z 1436) consistent with a glucosamine saccharide with two C 14 N-linked fatty acids.

Authors: Li, J; Cox, A; Martin, A; Hood, D; Moxon, R

• Journal: Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
• Pages: 69-70
• Publication date: 2002-01-01
• Language: English