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Far-field autofluorescence nanoscopy.

Abstract:

We demonstrate far-field optical imaging at the nanoscale with unlabeled samples. Subdiffraction resolution images of autofluorescent samples are obtained by depleting the ground state of natural fluorophores by transferring them to a metastable dark state and simultaneously localizing those fluorophores that are transiently returning. Our approach is based on the insight that nanoscopy methods relying on stochastic single-molecule switching require only a single fluorescence on-off cycle to ...

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Publication status:
Published

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Publisher copy:
10.1021/nl1027638

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Journal:
Nano letters More from this journal
Volume:
10
Issue:
10
Pages:
4249-4252
Publication date:
2010-10-01
DOI:
EISSN:
1530-6992
ISSN:
1530-6984
Language:
English
Keywords:
Pubs id:
pubs:222199
UUID:
uuid:b10fee5f-5d6c-4baa-b0e0-0425638180e1
Local pid:
pubs:222199
Source identifiers:
222199
Deposit date:
2012-12-19

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