We demonstrate far-field optical imaging at the nanoscale with unlabeled samples. Subdiffraction resolution images of autofluorescent samples are obtained by depleting the ground state of natural fluorophores by transferring them to a metastable dark state and simultaneously localizing those fluorophores that are transiently returning. Our approach is based on the insight that nanoscopy methods relying on stochastic single-molecule switching require only a single fluorescence on-off cycle to ...Expand abstract
- Publication status:
- Publisher copy:
- Copyright date:
Far-field autofluorescence nanoscopy.
If you are the owner of this record, you can report an update to it here: Report update to this record