Femtosecond near-infrared laser pulses elicit generation of reactive oxygen species in mammalian cells leading to apoptosis-like death.

Journal article

Two-photon excitation-based near-infrared (NIR) laser scanning microscopy is currently emerging as a new and versatile alternative to conventional confocal laser scanning microscopy, particularly for vital cell imaging in life sciences. Although this innovative microscopy has several advantages such as highly localized excitation, higher penetration depth, reduced photobleaching and photodamage, and improved signal to noise ratio, it has, however, recently been evidenced that high-power NIR l...

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Authors: Tirlapur, U; König, K; Peuckert, C; Krieg, R; Halbhuber, K

• Publication status: Published
• Journal: Experimental cell research
• Volume: 263
• Issue: 1
• Pages: 88-97
• Publication date: 2001-02-01
• DOI: 10.1006/excr.2000.5082
• EISSN: 1090-2422
• ISSN: 0014-4827
• Language: English
• Keywords: Cell Line; Animals; Immunohistochemistry; Kidney; DNA Fragmentation; Apoptosis; Epithelial Cells; Cell Nucleus; Microscopy; Reactive Oxygen Species; In Situ Nick-End Labeling; Lasers; Macropodidae; Cell Membrane; Oxidative Stress; Staining and Labeling