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A highly fluorescent DNA toolkit: synthesis and properties of oligonucleotides containing new Cy3, Cy5 and Cy3B monomers.

Abstract:

Cy3B is an extremely bright and stable fluorescent dye, which is only available for coupling to nucleic acids post-synthetically. This severely limits its use in the fields of genomics, biology and nanotechnology. We have optimized the synthesis of Cy3B, and for the first time produced a diverse range of Cy3B monomers for use in solid-phase oligonucleotide synthesis. This molecular toolkit includes phosphoramidite monomers with Cy3B linked to deoxyribose, to the 5-position of thymine, and to ...

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Publication status:
Published

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Publisher copy:
10.1093/nar/gks303

Authors


Gerowska, M More by this author
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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Organic Chemistry
Journal:
Nucleic acids research
Volume:
40
Issue:
14
Pages:
e108
Publication date:
2012-08-05
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
URN:
uuid:b00d4478-61cd-4d48-aa6b-66312def69bc
Source identifiers:
399980
Local pid:
pubs:399980

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