Conference item
A high-mass Q-TOF for the analysis of protein complexes
- Abstract:
- A tandem mass spectrometer (a modified Q-TOF) for the analysis of protein complexes was discussed. With nanoflow-ESI, large biomolecular assemblies were amenable to MS analysis in native environments. Using the instrument, complexes of the bacterial GroEL chaperonin were detected as intact molecular ions around m/z 12,000-14,000. It was found that isolation of single, well-resolved charge states and subsequent collision-induced dissociation (CID) leads to the formation of ions from individual subunits and subcomplexes.
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Authors
- Host title:
- Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
- Pages:
- 11-12
- Publication date:
- 2002-01-01
- Pubs id:
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pubs:323807
- UUID:
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uuid:afba2427-594c-45d6-a1e2-46510c63557f
- Local pid:
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pubs:323807
- Source identifiers:
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323807
- Deposit date:
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2013-02-20
- ARK identifier:
Terms of use
- Copyright date:
- 2002
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