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Characterisation and development of aspirin inducible biosensors in E. coli and SimCells

Abstract:

A simple aspirin-inducible system has been developed and characterised in E. coli by employing the Psal promoter and SalR regulation system originally from Acinetobacter baylyi ADP1. Mutagenesis at the DNA binding domain (DBD) and chemical recognition domain (CRD) of the SalR protein in A. baylyi ADP1 suggests that the effector free form SalRr could compete with the effector bound form SalRa, binding the Psal promoter and repress gene transcription. The induction of the Psal promoter was comp...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1128/AEM.02959-18

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Institution:
University of Oxford
Division:
MPLS Division
Department:
Engineering Science
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Engineering Science
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Engineering Science
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Publisher:
American Society for Microbiology Publisher's website
Journal:
Applied and Environmental Microbiology Journal website
Volume:
85
Issue:
6
Pages:
e02959-18
Publication date:
2019-01-18
Acceptance date:
2019-01-09
DOI:
EISSN:
1098-5336
ISSN:
0099-2240
Pubs id:
pubs:958207
URN:
uri:ad51942a-40d6-4843-94b5-afca4b63667e
UUID:
uuid:ad51942a-40d6-4843-94b5-afca4b63667e
Local pid:
pubs:958207

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