The role of cell membrane strain in sonoporation characterised by microfluidic-based single-cell analysis

Conference item

In the present study we have investigated the sonoporation dynamics in a single cell using a novel microfluidic-based approach. Our methodology has successfully addressed the biophysical mechanisms underlying US-induced cell membrane sonoporation by performing in situ measurement of localised cell membrane deformation, and simultaneous quantification of both intracellular calcium concentration ([Ca2+]i) and transmembrane transfer of extracellular membrane-impermeable probes. We have highlighted novel aspects of microbubble-cluster dynamics combined with localised cell membrane strain, which could be responsible for membrane permeabilisation and transmembrane pore formation correlated with the transduction of intracellular biochemical signals (i.e. [Ca2+]i influx) as a result of microbubble-cell interaction.

Authors: Bose, N; Carugo, D; Maiti, TK; Zhang, X; Chakraborty, S

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Chemical and Biological Microsystems Society
• Host title: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
• Volume: 3
• Pages: 1743-1745
• Publication date: 2011-12-01
• Event title: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2011)
• Event location: Seattle, Washington, USA
• Event website: https://www.microtasconferences.org/microtas2011/
• Event start date: 2011-10-02
• Event end date: 2011-10-06
• ISSN: 1556-5904
• ISBN: 9781618395955
• Language: English
• Keywords: microbubble-cluster; sonoporation; membrane deformation; biochemical signals; microfluidics