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Detection of PCR products from Mycobacterium avium subspecies Paratuberculosis using oligonucleotides containing multiple 2,4-dinitrophenyl reporter groups.

Abstract:

A pool of five oligonucleotides has been used to detect the pathogenic organism Mycobacterium avium subspecies paratuberculosis in PCR-amplified DNA from ruminants. The oligonucleotides were labelled at the 5'-end with three dinitrophenyl reporter groups and hybridised to the target DNA, which was fixed to a nylon membrane by ultraviolet irradiation. Colourimetric detection of the PCR product was carried out using an anti-DNP antibody conjugated to horseradish peroxidase or to alkaline phosph...

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Authors


Stevenson, K More by this author
Walker, CA More by this author
Grzybowski, J More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Organic Chemistry
Journal:
Biomedical peptides, proteins and nucleic acids : structure, synthesis and biological activity
Volume:
1
Issue:
1
Pages:
17-20
Publication date:
1994
ISSN:
1353-8616
URN:
uuid:ac2010f1-55e2-4acb-998a-ce93295f1aa6
Source identifiers:
400086
Local pid:
pubs:400086

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