T cell help mechanisms in the in vitro antibody response: the role of linked and non-linked recognition interactions.

Journal article

Mechanisms by which T and B lymphocytes co-operate in the in vitro secondary antibody response to trinitrophenyl (TNP)-conjugated soluble protein antigens were investigated. The generation of antibody responses was analyzed when haptenic and carrier determinants were either linked or non-linked. Ability to co-operate through each of these mechanisms was influenced by the experimental conditions employed, particularly the mode of preparation of the T cells and the antigen concentration used. Nylon wool filtration of T cells may deplete a T helper population involved in non-linked recognition interactions. High antigen concentrations favour the non-linked form of interaction whereas low antigen concentrations favour linked recognition interaction. These data suggest that at least two distinct co-operative mechanisms co-exist. However, experimental conditions can be defined under which either one mechanism predominates or where more than one mechanism is relevant.

Authors: Sullivan, C; Waldmann, H

• Publication status: Published
• Journal: Immunology
• Volume: 51
• Issue: 2
• Pages: 343-350
• Publication date: 1984-02-01
• EISSN: 1365-2567
• ISSN: 0019-2805
• Language: English
• Keywords: B-Lymphocytes; Lymphocyte Cooperation; Animals; Hemocyanin; Female; Antibody Formation; Male; Cells, Cultured; Spleen; Mice; T-Lymphocytes; Trinitrobenzenes; Mice, Inbred CBA; Concanavalin A; Antigens