Journal article
Transient transfection and expression of eukaryotic membrane proteins in Expi293F cells and their screening on a small scale: application for structural studies
- Abstract:
- Cancers, neurodegenerative and infectious diseases remain some of the leading causes of deaths worldwide. The structure-guided drug design is essential to advance drug development for these important diseases. One of the key challenges in the structure determination workflow is the production of eukaryotic membrane proteins (drug targets) of high quality. A number of expression systems have been developed for the production of eukaryotic membrane proteins. In this chapter, an optimized detailed protocol for transient transfection and expression of eukaryotic membrane proteins in Expi293F cells is presented. Testing expression and purification on a small scale allow optimizing conditions for sample preparation for downstream structural (cryo-EM) elucidation.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Accepted manuscript, pdf, 2.7MB, Terms of use)
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- Publisher copy:
- 10.1007/978-1-0716-1406-8_5
Authors
- Publisher:
- Springer Nature
- Journal:
- Structural Proteomics More from this journal
- Volume:
- 2305
- Pages:
- 105-128
- Series:
- Methods in Molecular Biology
- Publication date:
- 2021-05-06
- DOI:
- EISSN:
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1940-6029
- ISSN:
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1064-3745
- Pmid:
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33950386
- Language:
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English
- Keywords:
- Pubs id:
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1176493
- Local pid:
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pubs:1176493
- Deposit date:
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2021-05-25
- ARK identifier:
Terms of use
- Copyright holder:
- Springer.
- Copyright date:
- 2021
- Rights statement:
- © Springer Science+Business Media, LLC, part of Springer Nature 2021
- Notes:
- This is the accepted manuscript version of the article. The final version is available online from Springer at: https://doi.org/10.1007/978-1-0716-1406-8_5
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