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Conference item : Abstract

Quantifying binding affinities, kinetics and stoichiometry of biomolecular complexes with mass photometry

Abstract:

The characterisation of biomolecular complexes and their interactions is essential for understanding the function and regulation of cellular processes. Many of the existing biosensor techniques, despite their maturity, are often hampered by the requirement for surface immobilisation, introduction of tags and the inability to differentiate and quantify co-existing species. Here, we show that mass photometry based on interferometric scattering microscopy (iSCAT), can determine the binding affin...

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Publication status:
Published
Peer review status:
Reviewed (other)

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Publisher copy:
10.1016/j.bpj.2019.11.1137

Authors


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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
More from this funder
Name:
European Commission
Grant:
813152
819593
Publisher:
Biophysical Society
Journal:
Biophysical Journal More from this journal
Volume:
118
Issue:
3
Pages:
187A
Publication date:
2020-02-07
Acceptance date:
2020-01-31
Event title:
Biophysical Society Annual Meeting 2020 (BPS 2020)
Event location:
San Diego, California, USA
Event website:
https://www.biophysics.org/2020meeting
Event start date:
2020-02-15
Event end date:
2020-02-19
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
Language:
English
Keywords:
Subtype:
Abstract
Pubs id:
1094706
Local pid:
pubs:1094706
Deposit date:
2020-04-07

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