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Response of native and denatured hen lysozyme to high pressure studied by (15)N/(1)H NMR spectroscopy.

Abstract:

High-pressure (15)N/(1)H NMR techniques were used to characterize the conformational fluctuations of hen lysozyme, in its native state and when denatured in 8 M urea, over the pressure range 30--2000 bar. Most (1)H and (15)N signals of native lysozyme show reversible shifts to low field with increasing pressure, the average pressure shifts being 0.069 +/- 0.101 p.p.m. ((1)H) and 0.51 +/- 0.36 p.p.m. ((15)N). The shifts indicate that the hydrogen bonds formed to carbonyl groups or water molecu...

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Publication status:
Published

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Authors


Kamatari, YO More by this author
Akasaka, K More by this author
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Institution:
University of Oxford
Department:
Oxford, MPLS, Physics, Atomic and Laser Physics
Dobson, CM More by this author
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Journal:
European journal of biochemistry / FEBS
Volume:
268
Issue:
6
Pages:
1782-1793
Publication date:
2001-03-05
DOI:
EISSN:
1432-1033
ISSN:
0014-2956
URN:
uuid:aa57d8a5-10dc-42fa-88e9-c167ba8625c5
Source identifiers:
15868
Local pid:
pubs:15868

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