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Journal article

Agitation-free multiphoton microscopy of oblique planes.

Abstract:
The scanning two-photon fluorescence microscope produces optically sectioned images from the focal plane. It is sometimes desirable to acquire images from other planes of the specimen that are inclined with respect to the focal plane. In this Letter, we discuss the issues concerned with acquiring such images together with the effects of the inclination angle on image resolution and sectioning strength. To obtain images from oblique planes at high speed, a two-photon system was built wherein a novel optical system is used to provide aberration-free scanning.
Publication status:
Published

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Publisher copy:
10.1364/ol.36.000663

Authors


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Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Role:
Author


Journal:
Optics letters More from this journal
Volume:
36
Issue:
5
Pages:
663-665
Publication date:
2011-03-01
DOI:
EISSN:
1539-4794
ISSN:
0146-9592


Language:
English
Keywords:
Pubs id:
pubs:124561
UUID:
uuid:aa1ab29a-8004-4a94-924c-c14b94306c7d
Local pid:
pubs:124561
Source identifiers:
124561
Deposit date:
2013-11-17

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