- Abstract:
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Tethered enzymatic reactions are ubiquitous in signalling networks but are poorly understood. Here, a novel mathematical analysis is established for tethered signalling reactions in surface plasmon resonance (SPR). Applying the method to the phosphatase SHP-1 interacting with a phosphorylated tether corresponding to an immune receptor cytoplasmic tail provides 5 biophysical/biochemical constants from a single SPR experiment: two binding rates, two catalytic rates, and a reach parameter. Tethe...
Expand abstract - Publication status:
- Published
- Peer review status:
- Peer reviewed
- Version:
- Publisher's version
- Files:
-
-
(pdf, 1.4MB)
-
- Publisher copy:
- 10.1126/sciadv.1601692
-
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- Publisher:
- American Association for the Advancement of Science: Science Advances Publisher's website
- Journal:
- Science Advances Journal website
- Volume:
- 3
- Issue:
- 3
- Pages:
- Article: e1601692
- Publication date:
- 2017-01-01
- Acceptance date:
- 2017-02-09
- DOI:
- ISSN:
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2375-2548
- Pubs id:
-
pubs:681548
- URN:
-
uri:a9d9920d-886a-4f7c-900f-1e07a4b5225f
- UUID:
-
uuid:a9d9920d-886a-4f7c-900f-1e07a4b5225f
- Local pid:
- pubs:681548
- Paper number:
- 3
- Copyright holder:
- Goyette et al.
- Copyright date:
- 2017
- Notes:
-
Copyright © 2017 The Authors.
This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Journal article
Biophysical assay for tethered signaling reactions reveals tether-controlled activity for the phosphatase SHP-1
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