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Biophysical assay for tethered signaling reactions reveals tether-controlled activity for the phosphatase SHP-1

Abstract:

Tethered enzymatic reactions are ubiquitous in signalling networks but are poorly understood. Here, a novel mathematical analysis is established for tethered signalling reactions in surface plasmon resonance (SPR). Applying the method to the phosphatase SHP-1 interacting with a phosphorylated tether corresponding to an immune receptor cytoplasmic tail provides 5 biophysical/biochemical constants from a single SPR experiment: two binding rates, two catalytic rates, and a reach parameter. Tethe...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1126/sciadv.1601692

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Department:
Oxford, MSD, Pathology Dunn School
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Author
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Department:
Oxford, MPLS, Mathematical Institute
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Author
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Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
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Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
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Funding agency for:
Dushek, O
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Funding agency for:
Dushek, O
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Grant:
Sir Henry Dale Fellowship (098363)
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Grant:
Sir Henry Dale Fellowship (098363)
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Publisher:
American Association for the Advancement of Science: Science Advances Publisher's website
Journal:
Science Advances Journal website
Volume:
3
Issue:
3
Pages:
Article: e1601692
Publication date:
2017-01-01
Acceptance date:
2017-02-09
DOI:
ISSN:
2375-2548
Pubs id:
pubs:681548
URN:
uri:a9d9920d-886a-4f7c-900f-1e07a4b5225f
UUID:
uuid:a9d9920d-886a-4f7c-900f-1e07a4b5225f
Local pid:
pubs:681548
Paper number:
3

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