Journal article
Biophysical assay for tethered signaling reactions reveals tether-controlled activity for the phosphatase SHP-1
- Abstract:
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Tethered enzymatic reactions are ubiquitous in signalling networks but are poorly understood. Here, a novel mathematical analysis is established for tethered signalling reactions in surface plasmon resonance (SPR). Applying the method to the phosphatase SHP-1 interacting with a phosphorylated tether corresponding to an immune receptor cytoplasmic tail provides 5 biophysical/biochemical constants from a single SPR experiment: two binding rates, two catalytic rates, and a reach parameter. Tethe...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Version of record, pdf, 1.4MB)
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- Publisher copy:
- 10.1126/sciadv.1601692
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Authors
Funding
+ Wellcome Trust
More from this funder
Funding agency for:
Dushek, O
Grant:
Sir Henry Dale Fellowship (098363)
+ Royal Society
More from this funder
Funding agency for:
Dushek, O
Grant:
Sir Henry Dale Fellowship (098363)
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Bibliographic Details
- Publisher:
- American Association for the Advancement of Science: Science Advances Publisher's website
- Journal:
- Science Advances Journal website
- Volume:
- 3
- Issue:
- 3
- Article number:
- e1601692
- Publication date:
- 2017-01-01
- Acceptance date:
- 2017-02-09
- DOI:
- ISSN:
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2375-2548
- Source identifiers:
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681548
Item Description
- Pubs id:
-
pubs:681548
- UUID:
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uuid:a9d9920d-886a-4f7c-900f-1e07a4b5225f
- Local pid:
- pubs:681548
- Deposit date:
- 2017-02-23
Terms of use
- Copyright holder:
- Goyette et al
- Copyright date:
- 2017
- Notes:
-
Copyright © 2017 The Authors.
This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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