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Nematode-specific PCR primers for the 18S small subunit rRNA gene

Abstract:

A set of polymerase chain reaction primers were designed, which amplify a c. 1 kb fragment of the 18S ribosomal DNA gene, and are specific to the phylum Nematoda. These have proven useful in isolating nematode genes from samples mixed with other biological material, particularly with application to DNA barcoding. Optimal reaction conditions are described. These primers have successfully amplified the correct fragment from a wide phylogenetic range of nematodes, and have so far generated no se...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MPLS, Zoology
Lambshead, PJD More by this author
Journal:
MOLECULAR ECOLOGY NOTES
Volume:
5
Issue:
3
Pages:
611-612
Publication date:
2005-09-05
DOI:
EISSN:
1471-8286
ISSN:
1471-8278
URN:
uuid:a9d0b4d7-6c3e-4367-9cc9-8a28bef27244
Source identifiers:
244669
Local pid:
pubs:244669

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