- Abstract:
-
A set of polymerase chain reaction primers were designed, which amplify a c. 1 kb fragment of the 18S ribosomal DNA gene, and are specific to the phylum Nematoda. These have proven useful in isolating nematode genes from samples mixed with other biological material, particularly with application to DNA barcoding. Optimal reaction conditions are described. These primers have successfully amplified the correct fragment from a wide phylogenetic range of nematodes, and have so far generated no se...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1111/j.1471-8286.2005.01009.x
-
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- Journal:
- MOLECULAR ECOLOGY NOTES
- Volume:
- 5
- Issue:
- 3
- Pages:
- 611-612
- Publication date:
- 2005-09-05
- DOI:
- EISSN:
-
1471-8286
- ISSN:
-
1471-8278
- URN:
-
uuid:a9d0b4d7-6c3e-4367-9cc9-8a28bef27244
- Source identifiers:
-
244669
- Local pid:
- pubs:244669
- Language:
- English
- Keywords:
- Copyright date:
- 2005
Journal article
Nematode-specific PCR primers for the 18S small subunit rRNA gene
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