Journal article

Nematode-specific PCR primers for the 18S small subunit rRNA gene

Abstract:: A set of polymerase chain reaction primers were designed, which amplify a c. 1 kb fragment of the 18S ribosomal DNA gene, and are specific to the phylum Nematoda. These have proven useful in isolating nematode genes from samples mixed with other biological material, particularly with application to DNA barcoding. Optimal reaction conditions are described. These primers have successfully amplified the correct fragment from a wide phylogenetic range of nematodes, and have so far generated no sequences from any other organismal group. © 2005 Blackwell Publishing Ltd.

Publication status:: Published

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APA Style

Floyd, R., Rogers, A., Lambshead, P., & Smith, C. (2005). Nematode-specific PCR primers for the 18S small subunit rRNA gene. MOLECULAR ECOLOGY NOTES, 5(3), 611–612.

MLA Style

Floyd, R, et al. “Nematode-Specific PCR Primers for the 18S Small Subunit RRNA Gene.” MOLECULAR ECOLOGY NOTES, vol. 5, no. 3, 2005, pp. 611–12.

Chicago Style

Floyd, R, A Rogers, P Lambshead, and C Smith. 2005. “Nematode-Specific PCR Primers for the 18S Small Subunit RRNA Gene.” MOLECULAR ECOLOGY NOTES 5 (3): 611–12.
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Publisher copy:: 10.1111/j.1471-8286.2005.01009.x

Authors

+ Floyd, R More by this author

Role:: Author

+ Rogers, A More by this author

Institution:: University of Oxford
Division:: MPLS
Department:: Zoology
Role:: Author

+ Lambshead, P More by this author

Role:: Author

+ Smith, C More by this author

Role:: Author

Journal:: MOLECULAR ECOLOGY NOTES More from this journal
Volume:: 5
Issue:: 3
Pages:: 611-612
Publication date:: 2005-09-01
DOI:: 10.1111/j.1471-8286.2005.01009.x
EISSN:: 1471-8286
ISSN:: 1471-8278

Language:: English
Keywords:: DNA barcoding

specific amplification

185 ribosomal RNA (SSU)

nematodes
Pubs id:: pubs:244669
UUID:: uuid:a9d0b4d7-6c3e-4367-9cc9-8a28bef27244
Local pid:: pubs:244669
Source identifiers:: 244669
Deposit date:: 2013-11-16
ARK identifier:: ark:/29072/ora_a9d0b4d76c3e43679cc98a28bef27244

Terms of use

Copyright date:: 2005

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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