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R-spondins engage heparan sulfate proteoglycans to potentiate WNT signaling

Abstract:

R-spondins (RSPOs) amplify WNT signaling during development and regenerative responses. We previously demonstrated that RSPOs 2 and 3 potentiate WNT/β-catenin signaling in cells lacking leucine-rich repeat-containing G-protein coupled receptors (LGRs) 4, 5 and 6 (Lebensohn and Rohatgi, 2018). We now show that heparan sulfate proteoglycans (HSPGs) act as alternative co-receptors for RSPO3 using a combination of ligand mutagenesis and ligand engineering. Mutations in RSPO3 residues predicted to...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.7554/elife.54469

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Role:
Author
ORCID:
0000-0002-0687-5831
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Role:
Author
ORCID:
0000-0001-7865-2816
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Division:
MSD
Department:
NDM
Sub department:
Structural Biology
Role:
Author
ORCID:
0000-0002-4847-5529
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Role:
Author
ORCID:
0000-0002-1406-2566
Nederlandse Organisatie voor Wetenschappelijk Onderzoek More from this funder
Publisher:
eLife Sciences Publications Publisher's website
Journal:
eLife Journal website
Volume:
9
Publication date:
2020-05-20
Acceptance date:
2020-04-25
DOI:
EISSN:
2050-084X
Pmid:
32432544
Language:
English
Keywords:
Pubs id:
1106679
Local pid:
pubs:1106679
Deposit date:
2020-06-12

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