DNA helicases Sgs1 and BLM promote DNA double-strand break resection.

Journal article

A key cellular response to DNA double-strand breaks (DSBs) is 5'-to-3' DSB resection by nucleases to generate regions of ssDNA that then trigger cell cycle checkpoint signaling and DSB repair by homologous recombination (HR). Here, we reveal that in the absence of exonuclease Exo1 activity, deletion or mutation of the Saccharomyces cerevisiae RecQ-family helicase, Sgs1, causes pronounced hypersensitivity to DSB-inducing agents. Moreover, we establish that this reflects severely compromised DS...

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Authors: Gravel, S; Chapman, R; Magill, C; Jackson, S

• Journal: Genes and development
• Volume: 22
• Issue: 20
• Pages: 2767-2772
• Publication date: 2008-10-01
• DOI: 10.1101/gad.503108
• EISSN: 1549-5477
• ISSN: 0890-9369
• Language: English
• Keywords: RNA, Small Interfering; Recombination, Genetic; Humans; Osteosarcoma; Saccharomyces cerevisiae Proteins; DNA Helicases; Saccharomyces cerevisiae; Bloom Syndrome; DNA Repair; Mutation; Bone Neoplasms; RecQ Helicases; Plasmids; Microscopy, Fluorescence; DNA Breaks, Double-Stranded; Exodeoxyribonucleases; Transfection; Tumor Cells, Cultured