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High-speed fixed-target serial virus crystallography

Abstract:

We report a method for serial X-ray crystallography at X-ray free-electron lasers (XFELs), which allows for full use of the current 120-Hz repetition rate of the Linear Coherent Light Source (LCLS). Using a micropatterned silicon chip in combination with the high-speed Roadrunner goniometer for sample delivery, we were able to determine the crystal structures of the picornavirus bovine enterovirus 2 (BEV2) and the cytoplasmic polyhedrosis virus type 18 polyhedrin, with total data collection t...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/nmeth.4335

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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM; Structural Biology
Role:
Author
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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM; Structural Biology
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Biology
Oxford college:
Merton College
Role:
Author
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Funding agency for:
Ginn, H
Grant:
075491/04
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Funding agency for:
Sauter, N
Grant:
GM117126
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Funding agency for:
Stuart, D
Grant:
G1000099
European Cluster of Advanced Laser Light Sources More from this funder
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Publisher:
Nature Publishing Group Publisher's website
Journal:
Nature Methods Journal website
Volume:
14
Issue:
8
Pages:
805-810
Publication date:
2017-06-19
Acceptance date:
2017-05-15
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
Pmid:
28628129
Source identifiers:
702249
Language:
English
Keywords:
Pubs id:
pubs:702249
UUID:
uuid:a86c02c0-c9a0-4567-93a9-e997c87a91b3
Local pid:
pubs:702249
Deposit date:
2017-10-09

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