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Coarse-grained modeling of DNA–RNA hybrids

Abstract:
We introduce oxNA, a new model for the simulation of DNA–RNA hybrids that is based on two previously developed coarse-grained models—oxDNA and oxRNA. The model naturally reproduces the physical properties of hybrid duplexes, including their structure, persistence length, and force-extension characteristics. By parameterizing the DNA–RNA hydrogen bonding interaction, we fit the model’s thermodynamic properties to experimental data using both average-sequence and sequence-dependent parameters. To demonstrate the model’s applicability, we provide three examples of its use—calculating the free energy profiles of hybrid strand displacement reactions, studying the resolution of a short R-loop, and simulating RNA-scaffolded wireframe origami.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1063/5.0199558

Authors


More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Oxford college:
Somerville College
Role:
Author
ORCID:
0009-0005-3048-6245
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Role:
Author
ORCID:
0000-0002-3876-0190
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
ORCID:
0000-0002-2226-9524
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Sub department:
Theoretical Physics
Role:
Author
ORCID:
0000-0002-8438-910X


Publisher:
American Institute of Physics
Journal:
Journal of Chemical Physics More from this journal
Volume:
160
Issue:
11
Article number:
115101
Publication date:
2024-03-18
Acceptance date:
2024-01-26
DOI:
EISSN:
1089-7690
ISSN:
0021-9606


Language:
English
Pubs id:
2120429
Local pid:
pubs:2120429
Deposit date:
2025-04-29

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