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Hyaluronidase treatment of synovial fluid to improve assay precision for biomarker research using multiplex immunoassay platforms.

Abstract:

Synovial fluid (SF) is a difficult biological matrix to analyse due to its complex non-Newtonian nature. This can result in poor assay repeatability and potentially inefficient use of precious samples. This study assessed the impact of SF treatment by hyaluronidase and/or dilution on intra-assay precision using the Luminex and Meso Scale Discovery (MSD) multiplex platforms. SF was obtained from patients with knee osteoarthritis at the time of joint replacement surgery. Aliquots derived from t...

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Publication status:
Published

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Publisher copy:
10.1016/j.jim.2012.08.012

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDORMS
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDORMS
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDORMS
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDORMS
Role:
Author
Journal:
Journal of immunological methods More from this journal
Volume:
386
Issue:
1-2
Pages:
22-30
Publication date:
2012-12-01
DOI:
EISSN:
1872-7905
ISSN:
0022-1759
Language:
English
Keywords:
Pubs id:
pubs:350531
UUID:
uuid:a7feffe5-b221-4146-9cb1-8668a2a3a98f
Local pid:
pubs:350531
Source identifiers:
350531
Deposit date:
2013-11-16

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