Photoreceptor rhodopsin: structural and conformational study of its chromophore 11-cis retinal in oriented membranes by deuterium solid state NMR.

Journal article

Rhodopsin is the retinal photoreceptor responsible for visual signal transduction. To determine the orientation and conformation of retinal within the binding pocket of this membrane bound receptor, an ab initio solid state 2H NMR approach was used. Bovine rhodopsin containing 11-cis retinal, specifically deuterated at its methyl groups at the C19 or C20 position, was uniaxially oriented in DMPC bilayers. Integrity of the membranes and quality of alignment were monitored by 31P NMR. Analysis ...

Expand abstract

Authors: Gröbner, G; Choi, G; Burnett, I; Glaubitz, C; Verdegem, P

• Publication status: Published
• Journal: FEBS letters
• Volume: 422
• Issue: 2
• Pages: 201-204
• Publication date: 1998-01-01
• DOI: 10.1016/s0014-5793(97)01591-3
• EISSN: 1873-3468
• ISSN: 0014-5793
• Language: English
• Keywords: Protein Conformation; Animals; Binding Sites; Molecular Conformation; Rod Cell Outer Segment; Retinaldehyde; Dimyristoylphosphatidylcholine; Nuclear Magnetic Resonance, Biomolecular; Models, Molecular; Cattle; Deuterium; Rhodopsin; Lipid Bilayers