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OrthoFinder: scalable phylogenetic orthology inference for comparative genomics

Abstract:

Here, we present a major advance of the OrthoFinder method. This extends OrthoFinder’s high accuracy comparative genomic framework to provide substantially enhanced scalability and accuracy. Specifically, we show that enhanced phylogenetic delineation of orthogroups provides a 7% relative increase in orthogroup inference accuracy. We further demonstrate that a new gene assignment method substantially reduces overall runtime RAM usage without compromising accuracy. The latest version of OrthoFinder is available at https://github.com/OrthoFinder/OrthoFinder.

Publication status:
Published
Peer review status:
Not peer reviewed

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Preprint server copy:
10.1101/2025.07.15.664860

Authors

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Institution:
University of Oxford
Division:
MPLS
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Biology
Sub department:
CB BIOLOGY
Oxford college:
St John's College
Role:
Author
ORCID:
0000-0003-2392-6503
More by this author
Institution:
University of Oxford
Division:
MPLS
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Biology
Sub department:
CB BIOLOGY
Role:
Author
ORCID:
0000-0001-8583-5362


More from this funder
Funder identifier:
https://ror.org/029chgv08
Grant:
226598/Z/22/Z


Preprint server:
bioRxiv
Publication date:
2025-07-16
DOI:
Server owner:
Cold Spring Harbor Laboratory


Language:
English
Pubs id:
2247319
Local pid:
pubs:2247319
Deposit date:
2026-06-03
ARK identifier:

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