Journal article
SnoopLigase catalyzes peptide-peptide locking and enables solid-phase conjugate isolation
- Abstract:
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Simple, efficient reactions for connecting biological building-blocks open up many new possibilities. Here we have designed SnoopLigase, a protein that catalyzes site-specific transamidation, forming an isopeptide bond with more than 95% efficiency between two peptide tags, SnoopTagJr and DogTag. We initially developed these components by three-part splitting of the Streptococcus pneumoniae adhesin RrgA. The units were then engineered, guided by structure, bioinformatic analysis of...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
-
-
(Accepted manuscript, pdf, 1.4MB)
-
(Accepted manuscript, pdf, 1.5MB)
-
- Publisher copy:
- 10.1021/jacs.7b13237
Authors
Funding
AB Vista
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Engineering and Physical Sciences Research Council
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Biotechnology and Biological Sciences Research Council
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Bibliographic Details
- Publisher:
- American Chemical Society Publisher's website
- Journal:
- Journal of the American Chemical Society Journal website
- Volume:
- 140
- Issue:
- 8
- Pages:
- 3008–3018
- Publication date:
- 2018-02-06
- Acceptance date:
- 2018-02-06
- DOI:
- EISSN:
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1520-5126
- ISSN:
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0002-7863
Item Description
- Pubs id:
-
pubs:824437
- UUID:
-
uuid:a74fe491-90a4-4dc0-9eae-2c6b1f1c767a
- Local pid:
- pubs:824437
- Source identifiers:
-
824437
- Deposit date:
- 2018-02-14
Terms of use
- Copyright holder:
- American Chemical Society
- Copyright date:
- 2018
- Notes:
- Copyright © 2018 American Chemical Society. This is the accepted manuscript version of the article. The final version is available online from American Chemical Society at: https://doi.org/10.1021/jacs.7b13237
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