Synchrotron nano-FTIR spectroscopy for probing anticancer drugs at subcellular scale

Journal article

The cellular response to cisplatin was assessed in human osteosarcoma cells, using synchrotron-based (SR) Fourier Transform InfraRed nanospectroscopy (nano-FTIR) at the MIRIAM beamline B22 of Diamond Light Source (UK). This label-free mapping method delivered simultaneous morphological and biochemical information on a subcellular level (i.e. 100 s nanometer or better). Based on specific spectral biomarkers, the main biochemical constituents affected by the drug were identified at distinct locations within the cell´s inner body. Cisplatin was shown to have a noteworthy effect on proteins, mostly within the cytoplasm. A clear drug impact on cellular lipids was also observed. Within current literature on s-SNOM, this nanospectroscopy work represents a first successful application in life sciences providing full fingerprint nano-FTIR spectra across intact human cancer cells.

Authors: de Carvalho, LAEB; Cinque, G; de Carvalho, ALMB; Marques, J; Frogley, MD

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Nature Research
• Journal: Scientific Reports
• Volume: 14
• Issue: 1
• Article number: 17166
• Publication date: 2024-07-26
• Acceptance date: 2024-07-10
• DOI: 10.1038/s41598-024-67386-y
• EISSN: 2045-2322
• Language: English
• Keywords: Nanospectroscopy; Cisplatin; Osteosarcoma; Scattering-Scanning Near field Optical Microscopy (s-SNOM); Nano-Fourier Transform Infrared (nano-FTIR); Synchrotron Radiation (SR)