Ratiometric analysis of calcium mobilization

Journal article

One of the earliest events in lymphocyte activation is an increase in the concentration of cytosolic free Ca2+. Antigen recognition, chemotaxis, apoptosis and cell cycle progression are all associated with changes in cytosolic free Ca2+ levels. The kinetics, subcellular localization, and degree of Ca2+ mobilization can be assessed with fluorescent Ca2+ indicators. Ratiometric analysis of Ca2+ mobilization reduces errors due to variations in cell size and shape, indicator loading and instrument variability that can otherwise limit the usefulness of fluorescent Ca2+ indicators. The purposes of this paper are to summarize key characteristics of some useful Ca2+ -sensitive fluorescent dyes (Indo-1, Fura-2, Fluo-3, Fluo-4 and Fura Red) and to review published methods for the optimization of Ca2+ mobilization analysis with ratiometric indicators. Analysis of Ca2+ mobilization provides a rapid, quantitative readout of lymphocyte activation. © 2000 Elsevier Science Inc. All rights reserved.

Authors: Dustin, L

• Journal: Clinical and Applied Immunology Reviews
• Volume: 1
• Issue: 1
• Pages: 5-15
• Publication date: 2001-01-01
• DOI: 10.1016/S1529-1049(00)00002-7
• ISSN: 1529-1049
• Language: English
• Keywords: Ratiometric Ca2+ analysis; Lymphocyte activation; Ca2+; Flow cytometry; Signal transduction