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Novel high-resolution characterization of ancient DNA reveals C > U-type base modification events as the sole cause of post mortem miscoding lesions

Abstract:

Ancient DNA (aDNA) research has long depended on the power of PCR to amplify trace amounts of surviving genetic material from preserved specimens. While PCR permits specific loci to be targeted and amplified, in many ways it can be intrinsically unsuited to damaged and degraded aDNA templates. PCR amplification of aDNA can produce highly-skewed distributions with significant contributions from miscoding lesion damage and non-authentic sequence artefacts. As traditional PCR-based approaches ha...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1093/nar/gkm588

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Institution:
"University of Adelaide", "University of Oxford"
Research group:
Henry Wellcome Ancient Biomolecules Centre
Department:
Mathematical,Physical & Life Sciences Division - Zoology
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Institution:
University of Oxford
Research group:
Henry Wellcome Ancient Biomolecules Centre
Department:
Mathematical,Physical & Life Sciences Division - Zoology
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Institution:
"National Institute of Toxicology and Forensic Sciences, Canary Islands, Spain"
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Institution:
University of Reading
Department:
School of Animal and Microbial Sciences
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Institution:
University of Durham
Department:
Department of Archaeology
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Funding agency for:
Juan J. Sanchez
Publisher:
Oxford University Press Publisher's website
Journal:
Nucleic Acids Research Journal website
Volume:
35
Issue:
17
Pages:
5717-5728
Publication date:
2007-09-05
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
URN:
uuid:a6e59705-00f0-40c4-8892-42fa76edda9c
Local pid:
ora:3032
Language:
English
Keywords:
Subjects:

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