Unbiased yeast screens identify cellular pathways affected in Niemann–Pick disease type C

Journal article

Niemann–Pick disease type C (NPC) is a rare lysosomal storage disease caused by mutations in either the NPC1 or NPC2 genes. Mutations in the NPC1 gene lead to the majority of clinical cases (95%); however, the function of NPC1 remains unknown. To gain further insights into the biology of NPC1, we took advantage of the homology between the human NPC1 protein and its yeast orthologue, Niemann–Pick C–related protein 1 (Ncr1). We recreated the NCR1 mutant in yeast and performed screens to identify compensatory or redundant pathways that may be involved in NPC pathology, as well as proteins that were mislocalized in NCR1-deficient yeast. We also identified binding partners of the yeast Ncr1 orthologue. These screens identified several processes and pathways that may contribute to NPC pathogenesis. These included alterations in mitochondrial function, cytoskeleton organization, metal ion homeostasis, lipid trafficking, calcium signalling, and nutrient sensing. The mitochondrial and cytoskeletal abnormalities were validated in patient cells carrying mutations in NPC1, confirming their dysfunction in NPC disease.

Authors: Colaco, A; Fernandez-Suarez, ME; Shepherd, D; Diot, A; Morten, K

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Life Science Alliance
• Journal: Life Science Alliance
• Volume: 3
• Issue: 7
• Article number: e201800253
• Publication date: 2020-06-02
• Acceptance date: 2020-05-22
• DOI: 10.26508/lsa.201800253
• EISSN: 2575-1077
• Language: English
• Keywords: yeast; lysosomal storage disease; FFR; Ncr1; microtubule defects; NPC; mitochondria