Correlation of CD2 binding and functional properties of multimeric and monomeric lymphocyte function-associated antigen 3.

Dustin, M; Olive, D; Springer, T

Journal article

Correlation of CD2 binding and functional properties of multimeric and monomeric lymphocyte function-associated antigen 3.

Abstract:: LFA-3 was purified with an intact (mLFA-3) or an enzymatically removed membrane-anchoring domain (sLFA-3). Gel filtration and sucrose gradient sedimentation showed sLFA-3 to be a single highly glycosylated polypeptide chain in solution, while mLFA-3 formed micelles of 8 LFA-3 monomers. 125I-mLFA-3 bound to Jurkat T leukemic cell surface CD2 with much higher avidity than sLFA-3. mLFA-3 binding had characteristics of a multivalent interaction with cell surface CD2 and had an avidity of 1.5 nM for Jurkat cells and 12 nM for resting T cells. Two CD2 mAbs tested did not block mLFA-3 binding: 9-1 and CD2.1. These mAbs were tested in combination with LFA-3 for their ability to activate T cells. The combination of mLFA-3 and CD2.1 mAbs induced a rapid increase in cytosolic free Ca2+ in Jurkat cells which was proportional to mLFA-3 occupation of CD2 sites. sLFA-3 showed no activity in the Ca2+ flux assay. The combination of mLFA-3 and the CD2.1 mAbs significantly stimulated proliferation of PBMC. The combination of mLFA-3 and 9-1 mAbs was weakly or not mitogenic for the same cells. The combination of CD2.1 and sLFA-3 at concentrations up to 480 nM was not consistently mitogenic. Therefore, monomeric LFA-3/CD2 interaction appears to have a relatively low affinity, while multimeric LFA-3 binds with high avidity. T cell activation by binding of LFA-3 to CD2 appears to require occupation of 10(4) to 10(5) CD2 sites, which is likely to occur during adhesion, but is rare in receptor systems with soluble ligands.

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APA Style

Dustin, M., Olive, D., & Springer, T. (1989). Correlation of CD2 binding and functional properties of multimeric and monomeric lymphocyte function-associated antigen 3. Journal of Experimental Medicine, 169(2), 503–517.

MLA Style

Dustin, M., et al. “Correlation of CD2 Binding and Functional Properties of Multimeric and Monomeric Lymphocyte Function-Associated Antigen 3.” Journal of Experimental Medicine, vol. 169, no. 2, 1989, pp. 503–17.

Chicago Style

Dustin, M, D Olive, and T Springer. 1989. “Correlation of CD2 Binding and Functional Properties of Multimeric and Monomeric Lymphocyte Function-Associated Antigen 3.” Journal of Experimental Medicine 169 (2): 503–17.
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