MLST revisited: the gene-by-gene approach to bacterial genomics.

Journal article

Multilocus sequence typing (MLST) was proposed in 1998 as a portable sequence-based method for identifying clonal relationships among bacteria. Today, in the whole-genome era of microbiology, the need for systematic, standardized descriptions of bacterial genotypic variation remains a priority. Here, to meet this need, we draw on the successes of MLST and 16S rRNA gene sequencing to propose a hierarchical gene-by-gene approach that reflects functional and evolutionary relationships and catalo...

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Authors: Maiden, M; Jansen van Rensburg, M; Bray, J; Earle, S; Ford, SA

• Publication status: Published
• Journal: Nature reviews. Microbiology
• Volume: 11
• Issue: 10
• Pages: 728-736
• Publication date: 2013-10-01
• DOI: 10.1038/nrmicro3093
• EISSN: 1740-1534
• ISSN: 1740-1526
• Language: English
• Keywords: Genome, Bacterial; Genetic Variation; Bacterial Typing Techniques; Multilocus Sequence Typing; RNA, Bacterial; Alleles; Genomics; Databases, Genetic; Base Sequence; Bacteria; Evolution, Molecular; RNA, Ribosomal, 16S