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Analysis of TIMP expression and activity.

Abstract:
This chapter provides practical information on the assay of tissue inhibitor of metalloproteinase (TIMP) activity and background information enabling meaningful interpretation of the data. Protocols are given for assessing the presence of TIMPs in biological samples by immunoblotting and by virtue of their ability to inhibit matrix metalloproteinase (MMP) hydrolysis of protein substrates (reverse zymography) and synthetic fluorogenic substrates.

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Institution:
University of Oxford
Department:
Oxford, MSD, NDORMS
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, NDORMS
Journal:
Methods in molecular medicine
Volume:
135
Pages:
251-267
Publication date:
2007
EISSN:
1940-6037
ISSN:
1543-1894
URN:
uuid:a4c9f6b0-2d2d-425a-b3a8-a3536728ed7e
Source identifiers:
226943
Local pid:
pubs:226943

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