Journal article
Glutathione transferase photoaffinity labeling displays GST induction by safeners and pathogen infection
- Abstract:
- Glutathione transferases (GSTs) represent a large and diverse enzyme family involved in the detoxification of small molecules by glutathione conjugation in crops, weeds and model plants. In this study, we introduce an easy and quick assay for photoaffinity labeling of GSTs to study GSTs globally in various plant species. The small-molecule probe contains glutathione, a photoreactive group and a minitag for coupling to reporter tags via click chemistry. Under UV irradiation, this probe quickly and robustly labels GSTs in crude protein extracts of different plant species. Purification and mass spectrometry (MS) analysis of labeled proteins from Arabidopsis identified 10 enriched GSTs from the Phi(F) and Tau(U) classes. Photoaffinity labeling of GSTs demonstrated GST induction in wheat seedlings upon treatment with safeners and in Arabidopsis leaves upon infection with avirulent bacteria. Treatment of Arabidopsis with salicylic acid (SA) analog benzothiadiazole (BTH) induces GST labeling independent of NPR1, the master regulator of SA. Six Phi- and Tau-class GSTs that are induced upon BTH treatment were identified, and their labeling was confirmed upon transient overexpression. These data demonstrate that GST photoaffinity labeling is a useful approach to studying GST induction in crude extracts of different plant species upon different types of stress.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 3.7MB, Terms of use)
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- Publisher copy:
- 10.1093/pcp/pcad132
Authors
+ European Research Council
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- Funder identifier:
- https://ror.org/0472cxd90
- Grant:
- 101019324
- 616449
- Publisher:
- Oxford University Press
- Journal:
- Plant and Cell Physiology More from this journal
- Volume:
- 65
- Issue:
- 1
- Pages:
- 128-141
- Place of publication:
- Japan
- Publication date:
- 2023-10-31
- Acceptance date:
- 2023-10-23
- DOI:
- EISSN:
-
1471-9053
- ISSN:
-
0032-0781
- Pmid:
-
37924215
- Language:
-
English
- Pubs id:
-
1560441
- Local pid:
-
pubs:1560441
- Deposit date:
-
2025-04-29
- ARK identifier:
Terms of use
- Copyright holder:
- Font Farre et al
- Copyright date:
- 2023
- Rights statement:
- © The Author(s) 2023. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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