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Journal article

DNA is replicated at the nuclear cage.

Abstract:

Structures resembling nuclei are released when HeLa cells are lysed in a detergent and 2 M salt. These nucleoids, which lack any organized membrane structure, contain all the nuclear DNA packaged within a cage of RNA and protein. Their DNA is supercoiled so that the linear DNA must remain unbroken and looped during lysis. Following digestion with the restriction endonuclease, EcoRI, cages and associated DNA were filtered free of unattached DNA. Pulse-labelled (i.e. newly synthesized) DNA rema...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
Journal:
Journal of cell science
Volume:
46
Issue:
DEC
Pages:
365-386
Publication date:
1980-12-05
EISSN:
1477-9137
ISSN:
0021-9533
URN:
uuid:a4936235-5960-4475-b3d2-b79c3c8e1f3d
Source identifiers:
16922
Local pid:
pubs:16922

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