Journal article
Crystal structure of a bacterial photoactivated adenylate cyclase determined by serial femtosecond and serial synchrotron crystallography
- Abstract:
- OaPAC is a recently discovered blue-light-using flavin adenosine dinucleotide (BLUF) photoactivated adenylate cyclase from the cyanobacterium Oscillatoria acuminata that uses adenosine triphosphate and translates the light signal into the production of cyclic adenosine monophosphate. Here, we report crystal structures of the enzyme in the absence of its natural substrate determined from room-temperature serial crystallography data collected at both an X-ray free-electron laser and a synchrotron, and we compare these structures with cryo-macromolecular crystallography structures obtained at a synchrotron by us and others. These results reveal slight differences in the structure of the enzyme due to data collection at different temperatures and X-ray sources. We further investigate the effect of the Y6W mutation in the BLUF domain, a mutation which results in a rearrangement of the hydrogen-bond network around the flavin and a notable rotation of the side chain of the critical Gln48 residue. These studies pave the way for picosecond-millisecond time-resolved serial crystallography experiments at X-ray free-electron lasers and synchrotrons in order to determine the early structural intermediates and correlate them with the well studied picosecond-millisecond spectroscopic intermediates.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 17.6MB, Terms of use)
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- Publisher copy:
- 10.1107/s2052252524010170
Authors
+ National Research, Development and Innovation Office
More from this funder
- Funder identifier:
- https://ror.org/03g2am276
- Publisher:
- International Union of Crystallography
- Journal:
- IUCrJ More from this journal
- Volume:
- 11
- Issue:
- Pt 6
- Pages:
- 991-1006
- Place of publication:
- England
- Publication date:
- 2024-10-29
- Acceptance date:
- 2024-10-18
- DOI:
- EISSN:
-
2052-2525
- Pmid:
-
39470573
- Language:
-
English
- Keywords:
- Pubs id:
-
2054193
- UUID:
-
uuid_a338c8a2-cda8-47d1-985d-5df6fc84cecf
- Local pid:
-
pubs:2054193
- Source identifiers:
-
W4403851545
- Deposit date:
-
2026-01-30
- ARK identifier:
Terms of use
- Copyright date:
- 2024
- Rights statement:
- This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
- Licence:
- CC Attribution (CC BY)
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