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Analysis of compartmentalized cAMP: a method to compare signals from differently targeted FRET reporters.

Abstract:

Förster resonance energy transfer (FRET)-based reporters are important tools to study the spatiotemporal compartmentalization of cyclic adenosine monophosphate (cAMP) in living cells. To increase the spatial resolution of cAMP detection, new reporters with specific intracellular targeting have been developed. Therefore it has become critical to be able to appropriately compare the signals revealed by the different sensors. Here we illustrate a protocol to calibrate the response detected by di...

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Publisher copy:
10.1007/978-1-62703-622-1_5

Authors


Stangherlin, A More by this author
Koschinski, A More by this author
Zoccarato, A More by this author
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Journal:
Methods in molecular biology (Clifton, N.J.)
Volume:
1071
Pages:
59-71
Publication date:
2014
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
URN:
uuid:a2df1ab8-0e76-4f12-b236-cf0306f2195d
Source identifiers:
445903
Local pid:
pubs:445903

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