Cloning of mouse Cited4, a member of the CITED family p300/CBP-binding transcriptional coactivators: induced expression in mammary epithelial cells.

Journal article

The CITED family proteins bind to CBP/p300 transcriptional integrators through their conserved C-terminal acidic domain and function as coactivators. The 21-kDa mouse Cited4 protein, a novel member of the CITED family, interacted with CBP/p300 as well as isoforms of the TFAP2 transcription factor, coactivating TFAP2-dependent transcription. The cited4 gene consisted of only a single exon located on chromosome 4 at 56.5-56.8 cM flanked by marker genes kcnq4 and scml1. Expression of Cited4 protein was strong and selective in embryonic hematopoietic tissues and endothelial cells. In adult animals, Cited4 showed strong milk cycle-dependent induction in pregnant and lactating mammary epithelial cells. Strong induction of Cited4 expression was also observed in SCp2 mouse mammary epithelial cells during their prolactin-dependent in vitro differentiation. These results implied possible roles for Cited4 in regulation of gene expression during development and differentiation of blood cells, endothelial cells, and mammary epithelial cells.

Authors: Yahata, T; Takedatsu, H; Dunwoodie, S; Bragança, J; Swingler, T

• Publication status: Published
• Journal: Genomics
• Volume: 80
• Issue: 6
• Pages: 601-613
• Publication date: 2002-12-01
• DOI: 10.1006/geno.2002.7005
• EISSN: 1089-8646
• ISSN: 0888-7543
• Language: English
• Keywords: Transcription Factors; Animals; Embryo, Mammalian; Sequence Homology, Nucleic Acid; DNA, Complementary; Trans-Activators; Gene Expression Regulation, Developmental; Protein Binding; E1A-Associated p300 Protein; Sequence Alignment; COS Cells; Blotting, Northern; Nuclear Proteins; Epithelial Cells; Promoter Regions, Genetic; Mammary Glands, Animal; In Situ Hybridization; Female; DNA; Transcriptional Activation; Introns; Exons; Amino Acid Sequence; Cloning, Molecular; Sequence Homology, Amino Acid; Sequence Analysis, DNA; Mice; Base Sequence; Genes; Male; Molecular Sequence Data; Gene Expression; RNA, Messenger