Are Solid Particles Ready for Prime-Time Proteomics?

Journal article

We evaluate the performance of nonporous C-18 stationary phases in high-speed proteomics workflows. We employed two commercially available sub-2 μm nonporous particle (NPP) materials, ODS-IIIE (1.5 μm) and SOLAD (1.0 μm), to fabricate analytical columns using 150 μm internal diameter (i.d.) fused silica capillaries to ensure compatibility with nano-UHPLC and nano-HPLC pressure regimes. Using “long” NPP columns (15–25 cm) connected to a conventional nano-UHPLC, we found that both materials supported efficient peptide separations within the flow rate and pressure ranges typical of nano-UHPLC systems. Shorter columns, used with the 10- and 16 min Whisper Zoom 120 and Zoom 80 methods on the Evosep One HPLC, demonstrated competitive separation performance compared to columns packed with fully porous (FPP) and superficially porous particles (SPP), achieving full-width at half-maximum (FWHM) values below 2 s (Zoom 120) and 3 s (Zoom 80). DIA LC-MS/MS analysis of a human cell lysate digest demonstrated that NPP columns provided performance comparable to or slightly superior to those of FPP and SPP materials. These findings establish NPP-based columns as a viable and competitive alternative to FPP and SPP materials, particularly suited for high-throughput and high-sensitivity proteomics applications.

Authors: Kitano, ES; Demyanenko, Y; Mohammed, S

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: American Chemical Society
• Journal: Analytical Chemistry
• Volume: 97
• Issue: 34
• Pages: 18681-18688
• Publication date: 2025-08-20
• Acceptance date: 2025-08-15
• DOI: 10.1021/acs.analchem.5c03073
• EISSN: 1520-6882
• ISSN: 0003-2700
• Language: English