Separate processes mediate nucleotide-induced inhibition and stimulation of the ATP-regulated K(+)-channels in mouse pancreatic beta-cells.

Journal article

The mechanisms by which nucleotides stimulate the activity of the ATP-regulated K(+)-channel (KATP-channel) were investigated using inside-out patches from mouse pancreatic beta-cells. ATP produces a concentration-dependent inhibition of channel activity with a Ki of 18 microns. The inhibitory action of ATP was counteracted by ADP (0.1 mM) and GDP (0.2 mM) but not GTP (1 mM). Stimulation of channel activity was also observed when ADP, GDP and GTP were applied in the absence of ATP. The abilit...

Expand abstract

Authors: Bokvist, K; Ammälä, C; Ashcroft, F; Berggren, P; Larsson, O

• Publication status: Published
• Journal: Proceedings. Biological sciences / The Royal Society
• Volume: 243
• Issue: 1307
• Pages: 139-144
• Publication date: 1991-02-01
• DOI: 10.1098/rspb.1991.0022
• EISSN: 1471-2954
• ISSN: 0962-8452
• Language: English
• Keywords: Kinetics; Cells, Cultured; Animals; Adenosine Diphosphate; Islets of Langerhans; Guanosine Diphosphate; Mice; Guanosine Triphosphate; Adenosine Triphosphate; Electrophysiology; Potassium Channels; Membrane Potentials; Electric Conductivity; Mice, Inbred Strains