Journal article
Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa.
- Abstract:
- The selection of point mutation at codon 164 (from isoleucine to leucine) of the dihydrofolate reductase (DHFR) enzyme in Plasmodium falciparum is associated with high sulfadoxine /pyrimethamine (SP) resistance. Using the yeast expression system that allows the detection of dhfr allele present at low level, the presence of this mutation had previously been reported between 1998-1999 in Muheza, Tanzania, an area of high SP resistance. Eighty five P. falciparum isolates, obtained from the same area between 2002 and 2003, were analysed for the presence of Leu-164 mutation, using standard protocol based on PCR-RFLP. None of the isolates had the Leu-164 mutation.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 207.0KB, Terms of use)
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- Publisher copy:
- 10.1186/1475-2875-2-46
Authors
- Publisher:
- BioMed Central
- Journal:
- Malaria Journal More from this journal
- Volume:
- 2
- Issue:
- 1
- Article number:
- 46
- Publication date:
- 2003-12-01
- DOI:
- EISSN:
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1475-2875
- ISSN:
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1475-2875
- Language:
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English
- UUID:
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uuid:a137a56d-d54d-48e9-841f-d0c9a80b2389
- Local pid:
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pubs:38936
- Source identifiers:
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38936
- Deposit date:
-
2012-12-19
Terms of use
- Copyright holder:
- Imwong et al
- Copyright date:
- 2003
- Notes:
- © 2003 Ochong et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL (http://www.malariajournal.com/content/2/1/46).
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