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Preparing individual Drosophila egg chambers for live imaging.

Abstract:

Live cell imaging is an important technique applied to a number of Drosophila tissues used as models to investigate topics such as axis specification, cell differentiation and organogenesis (1). Correct preparation of the experimental samples is a crucial, often neglected, step. The goal of preparation is to ensure physiological relevance and to establish optimal imaging conditions. To maintain tissue viability, it is critical to avoid dehydration, hypoxia, overheating or medium deterioration...

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Publisher copy:
10.3791/3679

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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
Journal:
Journal of visualized experiments : JoVE
Issue:
60
Publication date:
2012-01-01
DOI:
EISSN:
1940-087X
ISSN:
1940-087X
Source identifiers:
316469
Language:
English
Keywords:
Pubs id:
pubs:316469
UUID:
uuid:a0cc379f-8a3f-4564-a65f-a3b741d357a7
Local pid:
pubs:316469
Deposit date:
2013-11-16

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