Real-time monitoring of enzymatic DNA hydrolysis by electrospray ionization mass spectrometry

Journal article

A fast and direct method for the monitoring of enzymatic DNA hydrolysis was developed using electrospray ionization mass spectrometry. We incorporated the use of a robotic chip-based electrospray ionization source for increased reproducibility and throughput. The mass spectrometry method allows the detection of DNA fragments and intact non-covalent protein-DNA complexes in a single experiment. We used the method to monitor in real-time single-stranded (ss) DNA hydrolysis by colicin E9 DNase a...

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Authors: van den Heuvel, R; Gato, S; Versluis, C; Gerbaux, P; Kleanthous, K

• Journal: Nucleic Acids Research
• Volume: 33
• Issue: 10
• Pages: 1-7
• Publication date: 2005-01-01
• DOI: 10.1093/nar/gni099
• EISSN: 1362-4962
• ISSN: 0305-1048
• Language: English