Book section
Activation of the non-canonical inflammasome in mouse and human cells
- Abstract:
- The non-canonical inflammasome is a signaling platform that allows for the detection of cytoplasmic lipopolysaccharides (LPS) in immune and non-immune cells. Upon detection of LPS, this inflammasome activates the signaling proteases caspase-4 and -5 (in humans) and caspase-11 (in mice). Inflammatory caspases activation leads to caspase self-processing and the cleavage of the pore-forming protein Gasdermin D (GSDMD). GSDMD N-terminal fragments oligomerize and form pores at the plasma membranes, leading to an inflammatory form of cell death called pyroptosis. Here, we describe a simple method to activate the non-canonical inflammasome in myeloid and epithelial cells and to measure its activity using cell death assay and immunoblotting.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Access Document
- Files:
-
-
(Preview, Accepted manuscript, pdf, 354.8KB, Terms of use)
-
- Publisher copy:
- 10.1007/978-1-0716-2144-8_5
Authors
- Publisher:
- Humana Press
- Pages:
- 51-63
- Series:
- Methods in Molecular Biology
- Series number:
- 2459
- Place of publication:
- New York
- Publication date:
- 2022-01-01
- DOI:
- EISSN:
-
1940-6029
- ISSN:
-
1064-3745
- Pmid:
-
35212953
- EISBN:
- 9781071621448
- ISBN:
- 9781071621431
- Language:
-
English
- Keywords:
- Pubs id:
-
1241535
- Local pid:
-
pubs:1241535
- Deposit date:
-
2022-03-10
- ARK identifier:
Terms of use
- Copyright holder:
- Bezbradica et al.
- Copyright date:
- 2022
- Rights statement:
- © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022.
- Notes:
- This is the accepted manuscript version of the chapter. The final version is available online from Springer at: https://doi.org/10.1007/978-1-0716-2144-8_5
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