Internet publication
Polyphenol oxidase silencing avoids protein crosslinking and enzymatic browning in Nicotiana benthamiana leaf extracts
- Abstract:
- Oxidation in extracts from agroinfiltrated leaves leads to browning and protein precipitation. Here we show that silencing of polyphenol oxidase (PPO) in Nicotiana benthamiana suppresses enzymatic browning and avoids crosslinking of endogenous proteins such as RuBisCo. PPO silencing does not impact plant growth or development and may also enhance transient gene expression. These results show that depletion of PPO activity could be highly beneficial for plant science applications and molecular pharming.
- Publication status:
- Published
- Peer review status:
- Not peer reviewed
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(Preview, Version of record, pdf, 2.2MB, Terms of use)
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- Publisher copy:
- 10.1101/2025.03.31.646341
Authors
+ European Research Council
More from this funder
- Funder identifier:
- https://ror.org/0472cxd90
- Grant:
- 101019324
+ Biotechnology and Biological Sciences Research Council
More from this funder
- Funder identifier:
- https://ror.org/00cwqg982
- Grant:
- BB/Y00969X/1
- Host title:
- bioRxiv
- Publication date:
- 2025-04-01
- DOI:
- Language:
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English
- Pubs id:
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2117802
- Local pid:
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pubs:2117802
- Deposit date:
-
2025-04-29
Terms of use
- Copyright holder:
- Mahadevan et al
- Copyright date:
- 2025
- Rights statement:
- The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
- Licence:
- CC Attribution (CC BY)
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