Osmoregulation of dimer resolution at the plasmid pJHCMW1 mwr locus by Escherichia coli XerCD recombination.

Journal article

Xer-mediated dimer resolution at the mwr site of plasmid pJHCMW1 is osmoregulated in Escherichia coli. Whereas under low-salt conditions, the site-specific recombination reaction is efficient, under high-salt conditions, it proceeds inefficiently. Regulation of dimer resolution is independent of H-NS and is mediated by changes in osmolarity rather than ionic effects. The low level of recombination at high salt concentrations can be overcome by high levels of PepA or by mutating the ARG box to a sequence closer to the E. coli ARG box consensus. The central region of the mwr core recombination site plays a role in regulation of site-specific recombination by the osmotic pressure of the medium.

Authors: Pham, H; Dery, K; Sherratt, D; Tolmasky, M

• Publication status: Published
• Journal: Journal of bacteriology
• Volume: 184
• Issue: 6
• Pages: 1607-1616
• Publication date: 2002-03-01
• DOI: 10.1128/jb.184.6.1607-1616.2002
• EISSN: 1098-5530
• ISSN: 0021-9193
• Language: English
• Keywords: Aspartic Acid Endopeptidases; Escherichia coli; Osmolar Concentration; Culture Media; Recombinases; Fungal Proteins; Plasmids; Mutation; Recombination, Genetic; Sodium Chloride; Dimerization; DNA Nucleotidyltransferases